Temporal and Spatial Determination of Germin Biosynthesis in Wheat Tissues
نویسندگان
چکیده
In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources.
منابع مشابه
Nucleotide sequence of a transcript encoding a germin-like protein that is present in salt-stressed barley (Hordeum vulgare L.) roots.
Salt stress alters the synthesis (in vivo labeling with [35S]Met of a large number of polypeptides in roots of CM72, a relatively salt-tolerant barley (Hordeum vulgare L.) cultivar (Hurkman and Tanaka, 1987; Hurkman et al., 1989). The most significant changes are related to the biosynthesis of three polypeptides related to germin, an approximately 130kD homopentameric protein comprised of subun...
متن کاملDetermination of Spatial-Temporal Correlation Structure of Troposphere Ozone Data in Tehran City
Spatial-temporal modeling of air pollutants, ground-level ozone concentrations in particular, has attracted recent attention because by using spatial-temporal modeling, can analyze, interpolate or predict ozone levels at any location. In this paper we consider daily averages of troposphere ozone over Tehran city. For eliminating the trend of data, a dynamic linear model is used, then some featu...
متن کاملOxalate oxidases and differentiating surface structure in wheat: germins.
Oxalate oxidases (OXOs) have been found to be concentrated in the surface tissues of wheat embryos and grains: germin is concentrated in root and leaf sheaths that surround germinated embryos; pseudogermin (OXO-psi) is concentrated in the epidermis and bracts that 'encircle' mature grains. Most strikingly, the epidermal accumulation of OXO-psi was found to presage the transition of a delicate '...
متن کاملWheat and barley seed system in Syria: How diverse are wheat and barley varieties and landraces from farmer’s fields?
"> The present study described the diversity of wheat and barley varieties andlandraces available in farmer’s fields in Syria using different indicators. Analysisof spatial and temporal diversity and coefficient of parentage along withmeasurements of agronomic and morphological traits were employed to explain thediversity of wheat and barley varieties or landraces grown by farmers in Syria.Farm...
متن کاملSpatio-temporal variation of wheat and silage maize water requirement using CGMS model
The Crop Growth Monitoring System (CGMS) has been applied for spatial biophysical resource analysis of Borkhar & Meymeh district in Esfahan province, Iran. The potentially suitable area for agriculture in the district has been divided into 128 homogeneous land units in terms of soil (physical characteristics), weather and administrative unit. Crop parameters required in the WOFOST simulatio...
متن کامل